Ver o código fonte de «PLCE1»

{{proteína
| Nome = 
| image = Protein PLCE1 PDB 2bye.png
| width = 
| caption ={{PDB|2bye}}
| Símbolo = PLCE1
| AltSymbols =NPHS3, PLCE, PPLC, fosfolipase C épsilon 1
| ATC_prefix=
| ATC_suffix=
| ATC_supplemental=
| CAS_number=
| CAS_supplemental=
| DrugBank=
| EntrezGene = 51196
| HGNCid =
| OMIM = 
| PDB =2BYE
| RefSeq = NP_001159451
| UniProt = Q9P212
| ECnumber = 
| Cromosoma = 10
| Brazo = 
| Banda = 10q23.33
| LocusSupplementaryData = (94.03 – 94.33 Mb)
}}A '''PLCE1''' ou '''1-fosfatidilinositol-4,5-bisfosfato fosfodiesterase épsilon-1''', tamén chamada  '''fosfoinosítido fosfolipase C épsilon 1''' ({{EC number|3.1.4.11}}), é un [[encima]] que nos humanos está codificado no [[xene]] ''PLCE1'' do [[cromosoma 10]].<ref name="pmid11022047">{{Cita publicación periódica | vauthors = Lopez I, Mak EC, Ding J, Hamm HE, Lomasney JW | title = A novel bifunctional phospholipase c that is regulated by Galpha 12 and stimulates the Ras/mitogen-activated protein kinase pathway | journal = J Biol Chem | volume = 276 | issue = 4 | pages = 2758–65 | date = May 2001 | pmid = 11022047 | pmc =  | doi = 10.1074/jbc.M008119200 }}</ref><ref name="pmid11022048">{{Cita publicación periódica | vauthors = Song C, Hu CD, Masago M, Kariyai K, Yamawaki-Kataoka Y, Shibatohge M, Wu D, Satoh T, Kataoka T | title = Regulation of a novel human phospholipase C, PLCepsilon, through membrane targeting by Ras | journal = J Biol Chem | volume = 276 | issue = 4 | pages = 2752–7 | date = May 2001 | pmid = 11022048 | pmc =  | doi = 10.1074/jbc.M008324200 }}</ref><ref name="entrez">{{Cita web | title = Entrez Gene: PLCE1 phospholipase C, epsilon 1| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=51196| accessdate = }}</ref>

Cataliza a reacción:<ref name=expasy>{{cita web |url=http://enzyme.expasy.org/EC/3.1.4.11 |título=ENZYME entry: EC 3.1.4.11 |dataacceso=23 de outubro de 2011|autor= |data= |obra= |idioma= |cita= }}</ref>

: 1-fosfatidil-1D-mio-inositol 4,5-bisfosfato + H<sub>2</sub>O <math>\rightleftharpoons</math> 1D-mio-inositol-1,4,5-trisfosfato + diacilglicerol

Está localizada na membrana do [[aparato de Golgi]]. Exprésase amplamente nos diferentes tecidos do corpo humano. É un [[isocima]] do encima [[fosfolipase C|fosfoinosítido fosfolipase C]]. A isoforma 1 está ausente nos [[leucocito]]s do [[sangue]] periférico (mentres que a isoforma 2 se expresa só na [[placenta]], [[pulmón]]s e [[bazo]]). A PLCE1 sobrexprésase durante os ataques cardíacos.<ref name=uniprotkb />

Os defectos en PLCE1 son causa da [[síndrome nefrótica]] tipo 3 (NPHS3), que consiste nun mal funcionamento da filtración glomerular renal que provoca [[proteinuria]], [[hipoalbuminemia]] e [[edema]].<ref name=uniprotkb />

== Función ==

A PLCE1 pertence á familia das [[fosfolipase]]s, as cales catalizan a [[hidrólise]] de polifosfoinosítidos como o [[fosfatidilinositol-4,5-bisfosfato]] (PtdIns(4,5)P2) para xerar os [[segundo mensaxeiro|segundos mensaxeiros]] [[inositol-trisfosfato 3-quinase|Ins(1,4,5)P3]] e [[diacilglicerol]]. Estes produtos inician unha fervenza de respostas intracelulares que ten como resultado o crecemento celular e [[diferenciación celular|diferenciación]] e [[expresión xénica]].<ref name="entrez"/>

Tamén regula as [[GTPase pequena|GTPases pequenas]] da [[superfamilia Ras|superfamilia RAS]] a través da actividade do factor de intercambio RAS-guanina que posúe. Como efector da [[proteína G]] pequena heterotrimérica, pode desempeñar un papel na supervivencia celular, crecemento celular, organización da [[actina]] e activación das [[célula T|células T]].<ref name=uniprotkb>{{cita web |url=http://www.uniprot.org/uniprot/Q9P212 |título=1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase epsilon-1 |dataacceso=23 de outubro de 2011|autor= |data= |obra= |idioma= |cita= }}</ref>

== Características ==
Contén os seguintes [[dominio proteico|dominios]]:<ref name=uniprotkb />

* O dominio que se asocia a RAS 1 está dexenerado e pode que non se una a HRAS.
* O dominio que se asocia a RAS 2 media na interacción con HRAS, RAP1A, RAP2A e RAP2B unidas a GTP e no recrutamento de HRAS na membrana celular.
* O dominio RAS-GEF ten actividade de [[factor intercambiador de nucleótido de guanina|factor intercambiador de guanina]] (GEF) con HRAS e RAP1A. Media na activación da [[MAPK|ruta das proteína quinases activadas por mitóxeno]] (MAPK).

Utiliza como [[cofactor]] o [[calcio]]. É activada polas subunidades GNA12, GNA13 e GNB1 a GNG2 da proteína G heterotrimérica. É activada por [[HRAS]], [[RAP1A]], [[RHOA]], [[RHOB]], [[RHOC]], [[RRAS]] e RRAS2. Así mesmo é activada polos [[receptor acoplado á proteína G|receptores acoplados á proteína G(s)]] ADRB2, PTGER1 e CHRM3 por medio da formación de [[AMP cíclico]] e [[RAP2B]]. É inhibida polos receptores acoplados á proteína G(i). Interacciona con IQGAP1 e [[RAP2A]].<ref name=uniprotkb />

== Notas ==
{{listaref}}

== Véxase tamén ==
=== Bibliografía ===
* {{Cita publicación periódica | vauthors = Jefferson JA, Shankland SJ | title = Familial nephrotic syndrome: PLCE1 enters the fray. | journal = Nephrol. Dial. Transplant. | volume = 22 | issue = 7 | pages = 1849–52 | year = 2007 | pmid = 17449496 | doi = 10.1093/ndt/gfm098 }}
* {{Cita publicación periódica | vauthors = Cefai D, Debre P, Kaczorek M, Idziorek T, Autran B, Bismuth G | title = Human immunodeficiency virus-1 glycoproteins gp120 and gp160 specifically inhibit the CD3/T cell-antigen receptor phosphoinositide transduction pathway. | journal = J. Clin. Invest. | volume = 86 | issue = 6 | pages = 2117–24 | year = 1991 | pmid = 1979339 | pmc = 329852 | doi = 10.1172/JCI114950 }}
* {{Cita publicación periódica | vauthors = Zauli G, Previati M, Caramelli E, Bassini A, Falcieri E, Gibellini D, Bertolaso L, Bosco D, Robuffo I, Capitani S | title = Exogenous human immunodeficiency virus type-1 Tat protein selectively stimulates a phosphatidylinositol-specific phospholipase C nuclear pathway in the Jurkat T cell line. | journal = Eur. J. Immunol. | volume = 25 | issue = 9 | pages = 2695–700 | year = 1995 | pmid = 7589147 | doi = 10.1002/eji.1830250944 }}
* {{Cita publicación periódica | vauthors = Chen P, Mayne M, Power C, Nath A | title = The Tat protein of HIV-1 induces tumor necrosis factor-alpha production. Implications for HIV-1-associated neurological diseases. | journal = J. Biol. Chem. | volume = 272 | issue = 36 | pages = 22385–8 | year = 1997 | pmid = 9278385 | doi = 10.1074/jbc.272.36.22385 }}
* {{Cita publicación periódica | vauthors = Mayne M, Bratanich AC, Chen P, Rana F, Nath A, Power C | title = HIV-1 tat molecular diversity and induction of TNF-alpha: implications for HIV-induced neurological disease. | journal = Neuroimmunomodulation | volume = 5 | issue = 3–4 | pages = 184–92 | year = 1998 | pmid = 9730685 | doi = 10.1159/000026336 }}
* {{Cita publicación periódica | vauthors = Haughey NJ, Holden CP, Nath A, Geiger JD | title = Involvement of inositol 1,4,5-trisphosphate-regulated stores of intracellular calcium in calcium dysregulation and neuron cell death caused by HIV-1 protein tat | journal = J. Neurochem. | volume = 73 | issue = 4 | pages = 1363–74 | year = 1999 | pmid = 10501179 | doi = 10.1046/j.1471-4159.1999.0731363.x }}
* {{Cita publicación periódica | vauthors = Nagase T, Kikuno R, Ishikawa K, Hirosawa M, Ohara O | title = Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro | journal = DNA Res. | volume = 7 | issue = 2 | pages = 143–50 | year = 2000 | pmid = 10819331 | doi = 10.1093/dnares/7.2.143 }}
* {{Cita publicación periódica | vauthors = Mayne M, Holden CP, Nath A, Geiger JD | title = Release of calcium from inositol 1,4,5-trisphosphate receptor-regulated stores by HIV-1 Tat regulates TNF-alpha production in human macrophages | url = https://archive.org/details/sim_journal-of-immunology_2000-06-15_164_12/page/n467 | journal = J. Immunol. | volume = 164 | issue = 12 | pages = 6538–42 | year = 2000 | pmid = 10843712 | doi = 10.4049/jimmunol.164.12.6538 }}
* {{Cita publicación periódica | vauthors = Jin TG, Satoh T, Liao Y, Song C, Gao X, Kariya K, Hu CD, Kataoka T | title = Role of the CDC25 homology domain of phospholipase Cepsilon in amplification of Rap1-dependent signaling | journal = J. Biol. Chem. | volume = 276 | issue = 32 | pages = 30301–7 | year = 2001 | pmid = 11395506 | doi = 10.1074/jbc.M103530200 }}
* {{Cita publicación periódica | vauthors = Schmidt M, Evellin S, Weernink PA, von Dorp F, Rehmann H, Lomasney JW, Jakobs KH | title = A new phospholipase-C-calcium signalling pathway mediated by cyclic AMP and a Rap GTPase | journal = Nat. Cell Biol. | volume = 3 | issue = 11 | pages = 1020–4 | year = 2001 | pmid = 11715024 | doi = 10.1038/ncb1101-1020 }}
* {{Cita publicación periódica | vauthors = Evellin S, Nolte J, Tysack K, vom Dorp F, Thiel M, Weernink PA, Jakobs KH, Webb EJ, Lomasney JW, Schmidt M | title = Stimulation of phospholipase C-epsilon by the M3 muscarinic acetylcholine receptor mediated by cyclic AMP and the GTPase Rap2B | journal = J. Biol. Chem. | volume = 277 | issue = 19 | pages = 16805–13 | year = 2002 | pmid = 11877431 | doi = 10.1074/jbc.M112024200 }}
* {{Cita publicación periódica | vauthors = Song C, Satoh T, Edamatsu H, Wu D, Tadano M, Gao X, Kataoka T | title = Differential roles of Ras and Rap1 in growth factor-dependent activation of phospholipase C epsilon | journal = Oncogene | volume = 21 | issue = 53 | pages = 8105–13 | year = 2002 | pmid = 12444546 | doi = 10.1038/sj.onc.1206003 }}
* {{Cita publicación periódica | vauthors = Bennasser Y, Badou A, Tkaczuk J, Bahraoui E | title = Signaling pathways triggered by HIV-1 Tat in human monocytes to induce TNF-alpha | journal = Virology | volume = 303 | issue = 1 | pages = 174–80 | year = 2003 | pmid = 12482669 | doi = 10.1006/viro.2002.1676 }}
* {{Cita publicación periódica | vauthors = Czyzyk J, Brogdon JL, Badou A, Henegariu O, Preston Hurlburt P, Flavell R, Bottomly K | title = Activation of CD4 T cells by Raf-independent effectors of Ras | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 100 | issue = 10 | pages = 6003–8 | year = 2003 | pmid = 12721365 | pmc = 156316 | doi = 10.1073/pnas.1031494100 }}
* {{Cita publicación periódica | vauthors = Lennartsson J, Wernstedt C, Engström U, Hellman U, Rönnstrand L | title = Identification of Tyr900 in the kinase domain of c-Kit as a Src-dependent phosphorylation site mediating interaction with c-Crk | journal = Exp. Cell Res. | volume = 288 | issue = 1 | pages = 110–8 | year = 2003 | pmid = 12878163 | doi = 10.1016/S0014-4827(03)00206-4 }}
* {{Cita publicación periódica | vauthors = vom Dorp F, Sari AY, Sanders H, Keiper M, Oude Weernink PA, Jakobs KH, Schmidt M | title = Inhibition of phospholipase C-epsilon by Gi-coupled receptors | journal = Cell. Signal. | volume = 16 | issue = 8 | pages = 921–8 | year = 2005 | pmid = 15157671 | doi = 10.1016/j.cellsig.2004.01.009 }}

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[[Categoría:Hidrolases]]